635. Effects of Mechanical Strain on Zmpste24-Deficient Prematurely Aged Muscle-Derived Stem Cells

Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells

In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...

Effects of Lithium Chloride on In Vitro Proliferation Rate of Rat Marrow- Derived Mesenchymal Stem Cells

Purpose: To evaluate the effects of lithium chloride on MSCs in vitro expansion rate. Materials and Methods: In this experimental study, bone marrow from 8 rats was plated at 5×105- cells/cm2 in the presence of 1,2,5,7 and 10 mM Lithium chloride and expanded through 3 passages. Twelve days after initiatial culture, the cells of different groups were stained with crystal violet in order to compa...

Effects of gamma radiation on adipose-derived mesenchymal stem cells of human breast tissue

Background: During radiation therapy, stromal cells surrounding the tumor (e.g mesenchymal stem cells) may affect the treatment outcomes. We aimed to investigate the effects of gamma radiation on the mRNA expression of cytokines, DNA damage and population doubling time (PDT) of adipose-derived mesenchymal stem cells (ASCs). Material and methods: ASCs were enzymatically extracted from breast tis...

Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions

Objective(s):Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet.  The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages. Material...

Mechanical Strain-mediated Syndecan Regulation and Its Effects on Adhesion of Vascular Smooth Muscle Cells